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. 2007 Dec 3;104(50):20019–20024. doi: 10.1073/pnas.0701738104

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© 2007 by The National Academy of Sciences of the USA

Freely available online through the PNAS open access option.

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Fig. 2. — The effect of VBP1 mutations on the VBP1–VirD2 interaction. Plasmids carrying vbp1 were introduced into the vbp triple-mutant GMV123 to complement the vbp function deficiency. The crude extracts (CE) of A. tumefaciens GMV123(pCBS52A) (S52A), GMV123(pCBY139A) (Y139A), GMV123(pCBH218A) (H218A), GMV123(pCBP239A) (P239A), or GMV123(pCBR260A) (R260A) were used for the pull-down assays using MBP-VirJ (VirJ) and MBP-VirD2 (VirD2). The pulled-down proteins were analyzed by Western blot using VBP1 antibody. VBP1* represents a VBP1 degradation product.