Fig. 6.

Plasmid pML122 DNA was coimmunoprecipitated by VBP1 antibody. Plasmid pML122 was introduced into A. tumefaciens A348 (WT), GMV123 (VBP⁻), and GMV123(pCBV1) (VBP1⁺); the bacteria were grown in the noninducing conditions (no vir gene expression). The crude extracts from the cells were coimmunoprecipitated by VBP1 antibody. VBP1 in the crude extracts (CE) and immunoprecipitates (IP) was detected by Western blot. The plasmid DNAs were amplified by PCR using the primers annealed to pML122 or pCBV1.