Fig. 1.

Envelope-independent release of VLPs generated by membrane-targeted PFV Gag proteins. (A) Schematic representation of the PFV Gag protein, indicating relevant landmarks [the PSAP L domain, a single lysine residue, glycine/arginine (G-R)-rich domains, and a proteolytic cleavage site near the C terminus of the ≈72-kDa protein]. Also shown are the various membrane-targeting peptides that were appended to the PFV Gag N terminus. (B) Virion production by 293T cells transfected with the PFVΔEnv proviral plasmid, either alone or along with a PFV Env expression vector. Virion and cell lysates were analyzed by Western blotting with αPFV human serum. (C) VLP production by 293T cells transfected with plasmids expressing PFV Gag appended with the indicated membrane-targeting signals.