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. 2007 Nov 28;104(50):20043–20048. doi: 10.1073/pnas.0709999104

Fig. 1.

Fig. 1.

Anti-HA antibodies specifically recognize the HA-PfROM1 protein in transgenic HA-PfROM1 3D7 parasites. (A) Western blot analysis of mature parasite extract probed with anti-HA antibody. Lane 1 shows transgenic HA-PfROM1–3D7 parasite protein extract and lane 2 shows parental 3D7 protein extract. Numbers on the left indicate positions of molecular weight markers in kilodaltons. The arrowhead shows the position of HA-PfROM1 detected in the transgenic parasite extract. (B) IFA analysis of HA-PfROM1 in a developing schizont (4 nuclei stage). (C) Merozoites in a segmenter probed with anti-HA rat mAb 3F10 (green) and the nuclear stain (blue, Upper) or with anti-AMA1 (red, Lower). Note in the individual merozoites anti-HA mAb staining appears to be localized on one side of the nucleus, with characteristic bulbous region (arrowheads) that is toward the apical end of the merozoites. The stalk region runs along one side of the nucleus. (Scale bar, 1 μm.)