Fig. 3.

HA-PfROM1 does not localize with markers of different known subcellular organelles. Staining with antibodies against different subcellular organelle markers is shown in the left-hand column. (A) MSP1, a merozoite surface marker; (B) Bip, an ER marker; (C) ERD2, a cis-Golgi marker; (D) ACP, an apicoplast marker; (E) cytochrome c, a mitochondrial marker. Corresponding costaining with rat anti-HA mAb 3F10 (A and C–E) or mouse anti-HA mAb 2C16 (B) together with the overlay between the red and green channels is shown in the subsequent columns. The right-hand column shows the corresponding DIC images. (Scale bar, 1 μm.)