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. 2007 Dec 5;104(50):20067–20072. doi: 10.1073/pnas.0706544105

Fig. 3.

Fig. 3.

Functional assays of the core-H sequences in transgenic mouse lines. (A) Transgenic constructs. A 187-bp segment containing the core-H sequence was examined for H activity on the MOR28 minigene, which is not expressed by itself. The mutant segment containing two mutated homeodomain sequences (#1 and #3) was also examined. The MOR28 minigene was tagged with IRES-gap-EYFP. (B) Coronal sections of the OE were imaged under fluorescent illumination for EGFP and EYFP. (Left) Expression of the endogenous MOR28 tagged with IRES-gap-EGFP (3) is shown. (Center) A large number of OSNs expressed the MOR28 minigene when the 187-bp sequence was attached. (Right) When the homeodomain sequences, #1 and #3, were mutated, no transgene-positive OSNs were detected. (C) Lateral views of the mouse OB. Glomerular formation for the endogenous MOR28 (Left) and the mutant core-H (Right) was analyzed as controls. (Center) Whole-mount fluorescence revealed groups of the EYFP-positive glomeruli, when the 187-bp core-H-containing sequence was attached to the MOR28 minigene. (D) Coronal sections of the OB. (Upper) Multiple glomerular structures were found for the MOR28 minigene with the 187-bp core-H-containing sequence. (Lower) Higher magnification is also shown. (Scale bars: B, 20 μm; C, 500 μm; D, 50 μm.)