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Generation of JP45 KO mice. (A) Targeting vector construction strategy. (B) Southern blot analysis of genomic DNA from ES cells of mutant mice according to Nishi et al. (27). (C) PCR analysis of genomic DNA from wild-type and JP45 KO mice. (D) SR R1, R2, and R4 fraction proteins (10 μg) separated by SDS/PAGE and stained with Coomassie BB (Left) and anti-JP45 Ab (Right). (E) Western blot analysis of SR fraction proteins with anti-SR proteins.
