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AvrRpt2 promotes auxin-induced gene expression in Arabidopsis protoplasts. Col-0 or Col-0 rps2-101c protoplasts were cotransfected with constructs encoding the indicated type III effector and a GH3-LUC reporter (29) and incubated for 3 h before treatment with 1 μM NAA. Expression of GH3-LUC was assayed 3 h after NAA treatment. The activities of GH3-LUC were detected in the absence (A) and presence (B) of NAA.
