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. 2007 Dec 3;104(50):20131–20136. doi: 10.1073/pnas.0704901104

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© 2007 by The National Academy of Sciences of the USA

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Fig. 4. — The avrRpt2-transgenic seedlings contain higher levels of free IAA. Free IAA levels were measured in shoot tissue of 7-day-old transgenic seedlings expressing avrRpt2 (filled bars) and nontransgenic (empty bars) seedlings. Results from experiments with Col-0 rps2 and Col-0 rps2-transgenic seedlings expressing pRPS2-avrRpt2 and No-0 rps2 and No-0 rps2-transgenic seedlings expressing p35S-avrRpt2 are shown. (A) Free IAA levels were normalized by fresh weight of the seedlings. (B) Data shown in A, with free IAA levels normalized for the number of seedlings assayed. Each bar represents the mean of three independent measurements ± SEM. The asterisks indicate a significant difference with P < 0.05, when compared with control tissue, based on the results of an unpaired one-tailed Student's t test. Similar results were obtained in two independent experiments, as well as in free IAA measurements of the root tissue of these seedlings (data not shown).