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. 1998 Apr 20;141(2):431–441. doi: 10.1083/jcb.141.2.431

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Immunohistochemical analysis of spinal anterior horn regions. (A–D) Semiquantitative direct immunofluorescence analysis of the localization of synaptic vesicle proteins in the spinal cord of wild-type mice (A and C) and homozygous mutants (B and D). High-magnification views of anterior horn of the T7 spinal cord stained with Cy5-labeled antisynaptophysin antibody (A and B) and Cy3.5-labeled anti-SV2 antibody (C and D). Some neuronal cell bodies are indicated with an asterisk, and some synaptic terminals are indicated with arrows. (E) Summary of the results of the quantitative analysis. Density, the density of the fluorescently labeled spots. Area, the mean area of the spots. Fluorescence, the upper tenth percentile value of the fluorescence intensity. Each value is shown in the format of mean ± SEM (n = 36 for wild-type mice, and 25 for mutant mice). Bar, 10 μm.

Immunohistochemical analysis of spinal anterior horn regions. (A–D) Semiquantitative direct immunofluorescence analysis of the localization of synaptic vesicle proteins in the spinal cord of wild-type mice (A and C) and homozygous mutants (B and D). High-magnification views of anterior horn of the T7 spinal cord stained with Cy5-labeled antisynaptophysin antibody (A and B) and Cy3.5-labeled anti-SV2 antibody (C and D). Some neuronal cell bodies are indicated with an asterisk, and some synaptic terminals are indicated with arrows. (E) Summary of the results of the quantitative analysis. Density, the density of the fluorescently labeled spots. Area, the mean area of the spots. Fluorescence, the upper tenth percentile value of the fluorescence intensity. Each value is shown in the format of mean ± SEM (n = 36 for wild-type mice, and 25 for mutant mice). Bar, 10 μm.