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. 1998 Apr 20;141(2):483–492. doi: 10.1083/jcb.141.2.483

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RacE null cells localize myosin II to a contractile ring in suspension conditions. Wild-type (A–C) and racE null (D–F) cells expressing GFP-myosin II were fixed in suspension cultures and stained with DAPI to visualize their nuclei. Under these conditions, wild-type cells concentrate myosin II in the cleavage furrow. These results differ from recently published observations that suggest myosin II does not localize to the furrow region of suspended cells (Neujahr et al., 1997). RacE null cells also localize myosin II in a contractile ring but their furrows never constrict to completion.