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. 1998 Apr 20;141(2):373–383. doi: 10.1083/jcb.141.2.373

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Analysis of Golgi contamination in isolated rat liver peroxisomal fractions. (A) Indicated amounts of isolated Golgi membranes and peroxisomes (Pox) primed with bovine brain cytosol in the presence of GTP-γS were subjected to SDS-PAGE and immunoblotting using an anti–β′-COP antibody. Note that the signal obtained with 10 μg of peroxisomes equals that of ∼2.5 μg of Golgi membranes. In B varying amounts of unprimed Golgi membranes and peroxisomes (Pox) were separated by SDS-PAGE and visualized by immunoblotting using antisera directed against Golgi P23 and Pmp69p, respectively. Note that the anti-P23 antiserum reliably recognizes 2 μg and even less of Golgi membranes and that even with 10 μg of peroxisomes no P23 immunoreaction product is observed.