Figure 6.

GTP-γS–dependent colocalization of coatomer and peroxisomes in SLO-permeabilized hepatocytes after density gradient fractionation. Permeabilized hepatocytes were incubated with rat liver cytosol and GTP-γS or GDP-βS for 30 min at 37°C. After homogenization of the recovered cells the postnuclear supernate was fractionated on a linear Nycodenz density gradient. The distribution of coatomer and peroxisomes was visualized by Western blotting using rabbit anti–β′-COP and anti-Pmp69p peptide antisera, respectively. Note that the anti–β′-COP antiserum in the Golgi fractions (fractions 7 and 8) recognizes a cross-reaction product of molecular weight slightly higher than β′-COP.