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. 1998 Apr 20;141(2):409–418. doi: 10.1083/jcb.141.2.409

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Binding of the COOH-terminal domain of MBS to the NH₂-terminal domain of ezrin. The in vitro–translated NH₂- or COOH-terminal domain of MBS was incubated with GST-ezrin (1–323 aa) or GST-immobilized beads. After the beads were washed, the bound proteins were eluted with GST-ezrin or GST by the addition of glutathione. The eluates were subjected to SDS-PAGE and vacuum-dried, followed by autoradiography. The in vitro–translated NH₂-terminal (arrow) and COOH-terminal (arrowhead) domains of MBS were shown. The results shown are representative of three independent experiments.