Table 1.

Interface characteristics of wild-type and affinity-matured cAb-RN05 and related proteins.

			Epitope			Paratope
Ligand	Kd (nM)	ΔG (kcal/mol)	# of atoms1	Ligand Efficiency	Area (Å2)	# of atoms1	Efficiency	Area (Å2)	SC2	Planarity
cAb-RN05
WT	23	10.3	46	0.22	589	47	0.22	613	0.76	2.13
matured	0.15	13.2	63	0.21	562	51	0.26	592	0.78	2.02
Other VHH
D3-L11 (1ZVY)	0.14	13.3	43	0.31	729	58	0.23	649	0.77	2.43
D2-L29 (1ZV5)	10	10.8	45	0.24	759	60	0.18	634	0.75	2.88
cAb-Lys3 (1JTT)	7.5	10.9	68	0.16	690	54	0.20	793	0.75	3.3
cAb-Lys2 (1RI8)	0.077	13.6	52	0.26	795	67	0.20	771	0.71	3.08
D2-L19 (1RJC)	3	11.5	59	0.19	741	60	0.19	644	0.79	2.88
D2-L24 (1ZVH)	80	9.5	63	0.15	528	51	0.19	593	0.82	1.65
cAb-Hu6 (1OP9)	0.7	12.3	45	0.27	556	43	0.29	577	0.76	1.61
Small molecule
SP4206-IL2R (1PY2)	68.8	9.6	44	0.22	422	30	0.32	572	0.77	2.01

¹Atoms within 4Å of the binding partner.

²Surface complementarity.²¹