Figure 2.

Elicitation of Hrp-dependent HR in leaves of N. clevelandii by E. coli DH5α carrying E. chrysanthemi hrp clusters that are either intact (pCPP2156, pCPP2416) or defective (pCPP2157, pCPP2368). N. clevelandii leaves were infiltrated with bacteria at a concentration of 5 × 10⁸ cfu/ml. Leaves were photographed 48 h after infiltration. Tissue collapse occurred within 24 h. The area below each number on the leaf was infiltrated with E. coli DH5α carrying the following constructs: 1, pCPP2156; 2, pCPP2156 and pAVRB-FLAG2; 3, pCPP2157; 4, pCPP2157 and pAVRB-FLAG2; 5, pCPP2416 and pAVRB-FLAG2; and 6, pCPP2368 and pAVRB-FLAG2.