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Differential secretion of AvrB-FLAG by E. coli DH5α carrying either a wild-type (pCPP2156) or mutant (pCPP2368) E. chrysanthemi hrp cluster. The supernatant fraction (S) was concentrated 7.5 times more than the cell pellet fraction (P). Lane 1, E. coli(pCPP2156, pAVRB-FLAG2); lane 2, E. coli(pCPP2368, pAVRB-FLAG2); lane 3, E. coli(pCPP2156, pAVRB-FLAG2); and lane 4, E. coli(pCPP2368, pAVRB-FLAG2).
