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Binding of CCUG 17874 (A) and 95–54 VacA (B) to HeLa and RK-13 cells. Cells were incubated at 0°C with increasing concentrations of cytotoxin, and the binding was revealed by flow cytometry by using m1- or m2-specific antiserum and fluorescein isothiocyanate-labeled anti-rabbit Ig. CCUG 17874 VacA (open-square), 95–54 VacA (closed-square). MFI, mean fluorescence intensity.
