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. 1998 Sep 7;142(5):1289–1299. doi: 10.1083/jcb.142.5.1289

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Cortical actin patches in act1-159 continue to move in the presence of LAT-A. LAT-A was added to 400 μM, and samples were taken during a 2-h time period and images were recorded to assess patch motility using GFP-ABP1. The cells pictured here had been growing in LAT-A–containing media for 86 min. The top white arrow points to a patch that moves away from the arrow towards the center of the cell. In the cell on the bottom right, the white arrow marks the starting position of an actin patch, and the black arrow points to its final destination. The low signal to noise ratio is due to decreased actin patch fluorescence intensity resulting from sequestration of actin monomers by LAT-A. Frames are 2-s apart. Bar, 5 μm.