Table I.
Expt. No. | Dys/αDB-1 | Dys/αDB-2 | Utr/αDB-1 | Utr/αDB-2 | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
−Ab | +Ab | fold** | −Ab | +Ab | fold | −Ab | +Ab | fold | −Ab | +Ab | fold | |||||||||||||
1 | 14,270* | 38,134 | 2.7 | 17,995 | 66,851 | 3.7 | 19,449 | 60,339 | 3.1 | 14,009 | 18,355 | 1.3 | ||||||||||||
2 | 884 | 1,918 | 2.2 | 1,057 | 4,411 | 4.2 | 33,097 | 134,637 | 4.0 | 47,288 | 44,523 | 0.9 | ||||||||||||
3 | 47,436 | 170,415 | 3.6 | 487 | 1,865 | 3.8 | 2,966 | 8,904 | 3.0 | 2,638 | 1,974 | 0.8 | ||||||||||||
4 | 9,493 | 28,209 | 3.0 | 20,182 | 67,344 | 3.3 | 3,840 | 13,792 | 3.6 | 1,343 | 1,472 | 1.1 | ||||||||||||
5 | 19,009 | 61,822 | 3.3 | 1,124 | 3,565 | 3.2 | 3,451 | 9,940 | 2.9 | 1,244 | 1,277 | 1.0 | ||||||||||||
3.0 | 3.6 | 3.3 | 1.0 | |||||||||||||||||||||
Avg. | (σ ± 0.5) | (σ ± 0.4) | (σ ± 0.4) | (σ ± 0.2) |
The band activities were quantified with ImageQuant software and are in units pixel. The positive band activities of at least twofold over the background pixel values were easily discernable.
fold, Fold difference between the band activities of the reaction containing the specific antibody and the control experiment (no antibody).