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. 1998 Sep 7;142(5):1181–1193. doi: 10.1083/jcb.142.5.1181

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Generation and characterization of monoclonal antibody 1D3. (a) SDS-PAGE of the proteins in the RNP extract from C. tentans tissue culture cells (lane 1), and of the proteins immunoprecipitated from the RNP extract by mAb 4F9 and used for subsequent immunization of BALB/c mice (lane 2). The protein recognized by mAb 4F9, hrp36, is indicated with an asterisk at lane 2. The position of molecular mass standards is shown to the left in kilodaltons. (b) Western blot analysis of the RNP extract probed with serum from an immunized mouse (lane 1), mAb 1D3 (lane 2), and negative control antibody (lane 3). The molecular mass standards are the same as in a.