Table II.
PCR Primers
| Primer | Sequence (5′→ 3′)* | |
|---|---|---|
| Knockout primers | ||
| MNS1forKan | aaacattgaaaaaggattctatgaagaactctgtcggtattcgatgaattcgagctc | |
| MNS1revKan | ccactatatagcacactaacctacaacgaccaacctgtggcgtacgctgcaggtcgac | |
| MNS1-68u | tgccaagaaacgaaagac | |
| MNS1+431L | cggataataaaccaccta | |
| ALG12forKan | aaaagagttgaataaagccattaaacaacgattcagttgacatcgatgaattcgagctc | |
| ALG12revKan | gctcgctatatattttattggaattgacgttagctattatcacgtacgctgcaggtcgac | |
| ALG12for | caaccttttaccagccgg | |
| KanMXu | gtattgatgttggacgag | |
| Primer for prc1-1 screen | ||
| CPY462u | ggatccggtcatcctttg | |
| CPY885L | ctgagtcaatgggtcagt | |
*
Bold face letters represent locus-specific sequence.