Table III.
Structure of N-linked Oligosaccharides in Mutant Yeast Strains
| Genotype | NLO after transfer | Final NLO structure* | Underglycosylation | References | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Δgls2 | Glc3Man9GlcNAc2 | Glc2Man8GlcNAc2 | (G2) | No | This study | |||||
| Δalg10 Δgls2 | Glc2Man9GlcNAc2 | Glc2Man8GlcNAc2 | (G2) | Yes | Jakob et al. (1998) | |||||
| Δalg8 Δgls2 | Glc1Man9GlcNAc2 | Glc1Man8GlcNAc2 | (G1) | Yes | Jakob et al. (1998) | |||||
| Δalg6 Δgls2 | Man9GlcNAc2 | Man8GlcNAc2 | (G0) | Yes | Jakob et al. (1998) | |||||
| Δmns1 | Glc3Man9GlcNAc2 | Man9GlcNAc2 | (M9) | No | Puccia et al. (1993) | |||||
| Wild-type‡ | Glc3Man9GlcNAc2 | Man8GlcNAc2 | (M8) | No | Jakob et al. (1998), Verostek et al. (1991, 1993), | |||||
| Ziegler and Trimble (1991) | ||||||||||
| Δalg6 | Man9GlcNAc2 | Man8GlcNAc2 | (M8) | Yes | Reiss et al. (1996) | |||||
| Δalg12 | Man7GlcNAc2 | Man7GlcNAc2 | (M7) | Yes | Burda, P., C.A. Jakob, J. Beinhauer, J.H. | |||||
| Hegemann, and M. Aebi, manuscript submitted for publication | ||||||||||
| Δalg9 | Man6GlcNAc2 | Man6GlcNAc2 | (M6) | Yes | Burda et al. (1996) | |||||
| Δalg3 | Man5GlcNAc2 | Man5GlcNAc2 | (M5) | Yes | Aebi et al. (1996) |
*
Oligosaccharide structure on glycoproteins (NLO) after endogenous glycosidase trimming. The term in parenthesis indicates the abbreviations used throughout the figures.
‡
The term wild-type is used with regard to oligosaccharide biosynthesis and trimming.