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. 1998 Sep 7;142(5):1357–1369. doi: 10.1083/jcb.142.5.1357

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The expression levels of focal contact-associated proteins in the α3-null keratinocytes are normal. (A) The levels of fluorescent-phalloidin–labeled F-actin in the α3-null and wild-type keratinocytes were the same. Student's t test showed no statistical difference in three experiments for a total of 11 samples. (B) Western blots of α3 integrin, actin, vinculin, talin, and α-actinin. The α3-subunit (α3, 150 kD) was absent in the α3-null keratinocytes. The levels of total actin (actin, 42 kD), vinculin (Vn, 110 kD), talin (Tn, 220 kD), and α-actinin (α-act, 100 kD) were all the same in the wild-type (+) and α3-null keratinocytes (−). Molecular weight markers represented in kD (kD). All cells were grown on vitrogen-coated plates.