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. 1998 Sep 7;142(5):1357–1369. doi: 10.1083/jcb.142.5.1357

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Adhesive and migratory behavior of α3-null and wild-type keratinocytes on various substrates. (A) Adhesion assays. Cell adhesion was measured as the percentage of cells adherent compared with the number of α3-null cells adherent to fibronectin (FN). A significantly higher number of α3-null keratinocytes adhered to fibronectin (FN), and collagen type IV (Coll IV) when compared with wild-type keratinocytes (P = 0.01 and P = 0.05, respectively). The numbers of wild-type and α3-null keratinocytes that adhered to either laminin 1 or laminin 5 were indistinguishable. When adhesion to laminin-5 was carried out in the presence of an α6β4 blocking antibody (GoH3), adhesion of the α3-null keratinocytes was significantly reduced whereas adhesion of the wild-type cells was not (P < 0.005). Results are an average of four experiments each done in triplicate. (B) Migration assays. Cell migration was measured as the area cleared by a single cell per hour. The α3-null keratinocytes migrated significantly further than wild-type cells on FN (P < 0.005) and Coll IV (P < 0.005). The migration of α3-null keratinocytes and wild-type cells on laminin 1 was not significantly different. In contrast, wild-type keratinocytes migrated significantly better than α3-null keratinocytes on laminin 5 (P < 0.005).