Table III.

Effect of mAbs to Adhesion Molecules on F-Actin Distribution in HUVEC

		Stress fiber–positive HUVEC (%)
		Medium (control)		CBL180 (anti–E-selectin)		4B9 (anti–VCAM-1)		RR1/1 (anti–ICAM-1)		MCA532 (anti–ICAM-1)		M89D3 (anti–PECAM-1)		CBL474 (anti–P-selectin)
Unstimulated HUVEC		15.1 ± 2.1		13.1 ± 2.1		6.2 ± 3.1		14.1 ± 1.8		11.6 ± 3.6		16.1 ± 2.7		15.2 ± 2.7
LPS-treated HUVEC		7.3 ± 1.8		58.5 ± 5.6*		67.2 ± 6.8*		12.9 ± 4.0		14.6 ± 2.7		12.3 ± 2.7		ND

Endothelial monolayers were pretreated for 5 h with medium or medium containing 0.5 μg/ml E. coli LPS. mAb or medium (control) was then added to the monolayers and the cells were incubated for 10 min at 37°C. The monolayers were then fixed and stained for F-actin. Coverslips were analyzed for HUVEC actin distribution by two observers. An average of 300–400 cells were examined on each coverslip. Values are means ± SE of three experiments.  

^* P < 0.01 (paired t test) compared to controls. ND, not determined.