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. 1998 Sep 7;142(5):1371–1379. doi: 10.1083/jcb.142.5.1371

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LFA-1–induced tyrosine phosphorylation in TAM2D2 cells is due to ZAP-70 activation. LFA-1 molecules were cross-linked using F(ab′) fragments of the rat mAb M17/4 against LFA-1 and anti-rat Ig antibodies and then immunoblotting was performed on cell lysates using the PY20 mAb against phosphotyrosine. (a) LFA-1 cross-linking induces tyrosine phosphorylation in TAM2D2 cells and WT cells overexpressing wild-type ZAP-70, whereas anti-rat Ig antibodies alone have no such effect in TAM2D2 cells. (b) Tyrosine phosphorylation occurs in TAM2D2 cells, but not in the DN22 and DN38 cells overexpressing dominant-negative ZAP-70 and in TAM2D2 cells treated with piceatannol.