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Budding Pattern of Haploid Cells
| Strain | Number of cells exhibiting nonaxial budding/600 cells | Percent nonaxial budding | ||
|---|---|---|---|---|
| CBY355 (WT) | 3 | 0.5 | ||
| CBY356 (erv14Δ) | 98 | 16.3 | ||
| CBY477 (erv14Δ/pRS316) | 93 | 15.5 | ||
| CBY508 (erv14Δ/pRS316-ERV14) | 7 | 1.2 | ||
| CBY409 (erv14Δ/pRS316-ERV14HA) | 2 | 0.3 | ||
| CBY462 (erv14Δ/p2μm-AXL2) | 40 | 6.7 |
Log phase cells with six bud scars or more were evaluated for nonaxial budding patterns. Cells that displayed one or more bud scars at opposite poles were scored as nonaxial.
