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. 1998 Aug 18;95(17):10257–10262. doi: 10.1073/pnas.95.17.10257

Figure 4.

Figure 4

Expression of caveolins 1 and 2 in DRG neurons. (A) Immunoblot analysis. Lysates were prepared from cultures of rat DRG neurons and divided into Triton-soluble (S) and Triton-insoluble (I) fractions. Caveolin-1 (pAb N-20) and caveolin-2 (mAb 65) specific antibody probes were then used to probe these cellular extracts by immunoblotting. Note that both caveolins 1 and 2 are expressed and behave as Triton-insoluble components. (B) RT-PCR analysis. Total RNA isolated from PC12 cells and DRG neurons was subjected to RT-PCR analysis by using primers directed against the known sequences of caveolins 1 and 2; NIH 3T3 cells served as a postitive control.