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. 2005 Jan 19;25(3):748–757. doi: 10.1523/JNEUROSCI.2399-04.2005

Figure 6.

Figure 6.

Quantification of rhodopsin-expressing cells after transfection of primary retinal cultures. Transfection and immunostaining were performed as described for Figure 4. The percentage of rhodopsin-expressing cells was scored at different time points. By 24 h after transfection, cells expressing mutant PRPF31 (either N371 or N256) show a significantly lower percentage of rhodopsin expression compared with cells expressing either GFP vector control or wild-type (WT) PRPF31-GFP fusion protein. The differences between the mutant group (either N371 or N256) and wild-type group (or GFP vector control) are statistically significant (*p < 0.005). The data were collected from three independent experiments.