Figure 7.

Mutant PRPF31 proteins do not affect synapsin expression. Primary retinal cells were transfected with plasmids expressing either wild-type or mutant PRPF31 as GFP-fusion proteins; wild-type (A1-A4), N371 (B1-B4), or N256 (C1-C4) PRPF31 proteins were detected in the transfected primary retinal cells by monitoring GFP fluorescence (A3-C3). Synapsin expression was detected by immunofluorescent staining (red fluorescence) using a monoclonal anti-synapsin antibody followed by the staining with the secondary antibody conjugated with Cy3 (A2-C2). The nuclear staining was performed with bis-benzamide (A4-C4). A1-C1 show the superimposed images in columns 2-4. The synapsin expression in cells expressing mutant PRPF31-GFP proteins shows no significant difference from those expressing the wild-type PRPF31-GFP protein (compare B2 and C2, with A2).