Figure 3.

Cosegregation of Prf mRNA overexpression with enhanced resistance to P. s. tomato T1. (a) RNA blot analysis showing expression of Prf in a population of plants homozygous for I1 and I2. Plants were screened for their response to virulent P. s. tomato T1 and avirulent P. s. tomato DC3000 after hand infiltration of leaves with bacteria at a concentration of 5 × 10⁴ cfu/ml. (b) The RNA blot from a probed with 18S rDNA as a loading control. Lane 1, wild-type; lane 2, prf-3 mutant; and lanes 3–16, I1/I2 plants. +, disease; −, no disease.