Figure 4.

Reconstitution of LHCP integration in stroma immunodepleted of cpSRP by adding back cpSRP54 and cpSRP43. Integration assays were performed as described (5). Stroma (30 μl), cpSRP54-immunodepleted stroma (30 μl), recombinant cpSRP43 (150 ng), and/or in vitro translated cpSRP54 (500 ng) were added as indicated. After 30 min at 25°C, thylakoids were treated with 0.1 mg/ml trypsin for 30 min at 0°C, followed by washing with 0.1 N NaOH. Each treatment is expressed relative to the positive control. The values indicated represent the average of three experiments. (Bars = SEM.)