Table 1.
Primer | Sequence (5′-3′) | Molecular weight | Tm | % G+C |
---|---|---|---|---|
3301 | TCGTAGCCAA | 3,013.0 | 32.1 | 50 |
3303 | TCACGATGCA | 3,013.0 | 32.6 | 50 |
IL0525 | CGGACGTCGC | 3,030.0 | 45.9 | 80 |
3307 | AGTGCTACGT | 3,044.0 | 22.7 | 50 |
B5 | TGCGCCCTTC | 2,956.0 | 45.6 | 70 |
B6 | TGCTCTGCCC | 2,956.0 | 40.2 | 70 |
B10 | CTGCTGGGAC | 3,045.0 | 33.6 | 70 |
B17 | AGGGAACGAG | 3,127.0 | 32.3 | 60 |
C19 | GTTGCCAGCC | 3,005.0 | 39.6 | 70 |
A2 | TGCCGAGCTG | 3,045.0 | 42.4 | 70 |
A9 | GGGTAACGCC | 3,054.0 | 38.7 | 70 |
A10 | GTGATCGCAG | 3,069.0 | 29.8 | 60 |
C2 | GTGAGGCGTC | 3,085.0 | 33.4 | 70 |
A17 | GAAACGGGTG | 3,118.1 | 34.5 | 60 |
A8 | GTGACGTAGG | 3,109.1 | 22.9 | 60 |