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. 1999 Aug 9;146(3):609–620. doi: 10.1083/jcb.146.3.609

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Perinuclear GLUT4 and γ-adaptin staining are mostly separate. 3T3-L1 adipocytes were stained for GLUT4 (red) and γ-adaptin (green) before deconvolution microscopy. The images shown are the center cross-sections from the deconvolved set of images. GLUT4 staining is shown in a and d; γ-adaptin staining is shown in b and e. Merged images are shown in c and f. a–c show staining of unstimulated 3T3-L1 adipocytes, and d–f show staining of insulin-stimulated 3T3-L1 adipocytes. Arrowheads indicate plasma membrane localization of GLUT4 in cells treated with insulin. Bar, 10 μm.