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. 1999 Aug 9;146(3):621–630. doi: 10.1083/jcb.146.3.621

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Developmental expression of the RyR1 and RyR3 isoforms in murine diaphragm and hind limb muscles. Microsomes were prepared from hind limb and diaphragm muscles of 2-d-old (2d), 15-d-old (15d), and adult mice (60d). 3 and 10 μg of microsomal vesicles were loaded for RyR1 and RyR3 immunodetection, respectively. In all experiments, 10 and 30 μg from bovine diaphragm were loaded as control (ctr) for RyR1 and RyR3 immunodetection, respectively. Western blot analysis was performed, as described in the text.