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. 1999 Aug 9;146(3):559–572. doi: 10.1083/jcb.146.3.559

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Intracellular localization of Rpp38. Swiss 3T3 fibroblasts were transiently transfected (see Materials and Methods) with pEGFP-Rpp38 (A and B) or pEGFP-C1 (C and D). 48 h after transfection, living cells were examined with a confocal microscope. Colocalization of GFP-Rpp38 in the nucleolus with the protein B23 in transfected HeLa cells is demonstrated by indirect immunofluorescence analysis (E–H). (I–K) Immunofluorescence of endogenous Rpp38 protein in untransfected 3T3 fibroblasts using anti-Rpp38 antibodies showing uniformly stained nucleoli. Images of DIC (A, C, E, and I), GFP (B, D, and G), B23 (F), and anti-Rpp38 (J) are shown. H is an overlay of F and G, and K is an overlay of I and J. No specific signal above autofluorescence was seen when control sera of rabbits were tested (not shown). Arrows point to nucleoli. Bars: B, D, and K, 5 μm; H, 2.5 μm.