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Subnucleolar localization of GFP-Rpp29 and identification of NS29. Swiss 3T3 fibroblasts were transiently transfected with pEGFP-Rpp29 (A–C) or pEGFP-Rpp29(52-85) (D–F). 48 h after transfection, localization of the fusion proteins was determined by confocal microscopy. DIC (left) GFP (middle), and overlay (right) images are shown. Arrows indicate nucleoli. Bars: C and F, 3.3 and 5 μm, respectively.
