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. 1999 Aug 9;146(3):559–572. doi: 10.1083/jcb.146.3.559

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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GFP-Rpp29 is localized in nucleoli and coiled bodies. HeLa cells were transfected for 48 h with pEGFP-Rpp29 (A–D) or pEGFP-Rpp14 (E–H), and then immunostained for p80-coilin in indirect immunofluorescence analysis. DIC (A and E), p80-coilin (B and F, red), GFP (C and G, green), and the overlays of B over C and F over G are shown in D and H, respectively. Images in A–D and E–H were acquired at the same confocal plane. The GFP fluorescent signal in C and D was enhanced to highlight the punctate staining seen in the nucleoplasm. Coiled bodies are indicated by arrows. Inserts seen in A–D represent higher magnification of coilin-immunostained coiled bodies in the periphery to two nucleoli of HeLa cells expressing GFP-Rpp29. All images were obtained during the same experimental observation. Bars: D and H, 2.5 μm.