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. 1999 Aug 9;146(3):559–572. doi: 10.1083/jcb.146.3.559

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Subnucleolar localization patterns of Rpp14. 3T3 fibroblasts were subjected to indirect immunofluorescent analysis using anti-Rpp14 antibodies (A–C). C is an overlay of A and B. Fibroblasts transfected for 48 h with pEGFP-Rpp14 were examined under confocal microscope (D–F) before fixation and immunofluorescence analysis with anti-Rpp29 antibodies (G–I). F is an overlay of DIC (not shown) and E; two cells with high fluorescent signal are indicated by arrowheads. I is an overlay of G and H. Intense yellow color is seen in nucleoli. Bars: C and D, 3.3 μm; F and I, 10 μm.