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. 1999 Aug 9;146(3):543–558. doi: 10.1083/jcb.146.3.543

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Three-dimensional visualization of transcription sites in permeabilized 3T3 cells. (A) 0.3-μm optical sections were reconstructed to form a three-dimensional image. Transcription sites are clustered in some regions of the cell nucleus, whereas others have no transcription activity. (B) A portion of A is further enlarged. Three-dimensional observations reveal clustering of individual sites into separate higher order domains. These separate higher order domains, in turn, are arranged in a pattern that resembles a networklike organization. (C) The centers of gravity (x, y, z) of transcription sites were determined by an algorithm after segmentation and displayed in three dimensions. Two specific transcription sites were pinpointed by their x, y, z coordinates and highlighted in purple and green. (D) A portion of the image of the centers of gravity is further enlarged.