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. 1999 Mar 22;144(6):1203–1218. doi: 10.1083/jcb.144.6.1203

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Ark1p-GFP and Prk1p-GFP localization to cortical patches is reduced in abp1Δ cells, but not in sla2Δ, sac6Δ, srv2Δ, or rvs167Δ cells. (A) Actin and (B) Prk1p-GFP localization in (i) sac6Δ, (ii) abp1Δ, (iii) rvs167Δ, (iv) srv2Δ, and (v) sla2Δ cells. (C) Actin and (D) Ark1p-GFP localization in (i) sac6Δ, (ii) abp1Δ, (iii) rvs167Δ, (iv) srv2Δ, and (v) sla2Δ cells. sac6Δ (DDY318), abp1Δ (DDY322), rvs167Δ (DDY950), srv2Δ (DDY952), or sla2Δ (DDY1166) cells were transformed with either pDD554 (GFP-Prk1p) or pDD555 (GFP-Ark1p), expression was induced for 8–12 h, and actin and GFP localization was examined. GFP-Ark1p and GFP-Prk1p are mostly found in the cytoplasm when expressed in abp1Δ cells. In only a small population of cells (<10%) can faint cortical patches of GFP-Ark1p or GFP-Prk1p be detected. Bar, 5 μm.