Figure 3.
(Top) Successive layers of the Golgi model, each viewed from its cis side. cis-ER, the ER on the cis side; ERGIC, the ERGIC layer; C1–C7, the cisternae in cis-to-trans order; trans-ER, the ER on the trans side. C6′ is a partial cisterna, positioned between C6 and C7, which we interpret as ER. The starred object in C5 represents a membrane tubule of unknown origin. The noncompact region (NCR) displays all the elements lying between the two compact regions but not connected to either. All coated vesicles budding from cisternal margins are highlighted with stippling. The trans-most cisterna, C7, is the only one that is budding clathrin-coated vesicles (yellow stippling). All fenestrae and holes within each cisterna are included. (Bottom) Stereo image of the complete model including the free vesicles that surround the stacks (not shown in Fig. 1). Yellow, blue, and green spheres represent clathrin-coated, non–clathrin-coated, and noncoated vesicles, respectively. Bars, 250 nm.