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. 1999 Mar 22;144(6):1113–1122. doi: 10.1083/jcb.144.6.1113

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Differential effects of labeling with [¹⁴C]thymidine and [³H]thymidine on MPCs in vitro. H-2K^bclone 18 MPCs were cultured for 16 h in the presence of 0.25 μCi/ml of either [¹⁴C]thymidine or [³H]thymidine, and then replated at 500 cells/cm². The numbers of cells in cultures of [¹⁴C]thymidine–labeled (filled circles), [³H]thymidine–labeled (filled squares), and control unlabeled (open circles) cells were counted over 4 d. Each point is the mean of three cultures ± 1 SD. The growth of MPCs precultured in presence of equivalent concentrations of unlabeled thymidine (4.6 μM for [¹⁴C]thymidine and 50 pM for [³H]thymidine) was indistinguishable from the control, unlabeled cells at thymidine concentrations of standard medium (data not shown).