Table I.
Movement of Listeria monocytogenes in Platelet Extracts: Role of the EVH1 Domain of Proteins of the Ena/VASP Family
| Experiment | Conditions | Rate of movement | % of bacteria surrounded by actin clouds | |||
|---|---|---|---|---|---|---|
| 1 | Control | 4.5 ± 0.6 (12) | ||||
| 0 at t = 10 min | ||||||
| + 2.5 μM GST-EVH1 (VASP) | 3.9 ± 0.7 (4) at t = 30 min | |||||
| 1.6 ± 0.5 (4) at t = 120 min | ||||||
| + 2.5 μM GST-EVH1 (Mena) | 0 at t = 10 min | |||||
| + 2.5 μM GST-EVH1 (Evl) | 0 at t = 10 min | |||||
| 2 | Control | 5.4 ± 0.4 (11) | 27 (223) | |||
| + 0.8 μM GST-Pro(ActA) | 0 | 15 (324) | ||||
| + 1.6 μM GST-Pro(ActA) | 0 | 16 (121) |
Numbers in parentheses indicate the number of measurements from which the average value was calculated. The control was performed by adding to the extract a volume of PBS equal to the volume of reagent added to the sample.