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. 1999 Mar 22;144(6):1285–1294. doi: 10.1083/jcb.144.6.1285

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Caveolin is required for β1 integrin–dependent adhesion to fibronectin. (A) Lysates of 293 cells (clones 6, 9, 31, and 32) expressing antisense caveolin before (−) and after (+) cadmium (CdCl₂) induction were immunoblotted for caveolin-1 and reprobed for cortactin as a control (left). Relative caveolin and cortactin levels among the clones were quantified by densitometry (right) and confirmed in three separate experiments. (B) Clones 31 and 32 with marked depletion of caveolin (A) did not adhere to fibronectin, whereas clones 6 and 9 and control 293 cells adhered avidly. (C) FACS^® analysis of β1, αv, and α5β1 integrin expression on control clone 6, and antisense clone 31 which has a markedly reduced caveolin level. B and C were performed three times with similar results. (D) Photographs of phase images (a and b) and paxillin immunostaining (c and d) of clones 6 and 31 after plating on fibronectin and subsequent induction with CdCl₂.

Caveolin is required for β1 integrin–dependent adhesion to fibronectin. (A) Lysates of 293 cells (clones 6, 9, 31, and 32) expressing antisense caveolin before (−) and after (+) cadmium (CdCl₂) induction were immunoblotted for caveolin-1 and reprobed for cortactin as a control (left). Relative caveolin and cortactin levels among the clones were quantified by densitometry (right) and confirmed in three separate experiments. (B) Clones 31 and 32 with marked depletion of caveolin (A) did not adhere to fibronectin, whereas clones 6 and 9 and control 293 cells adhered avidly. (C) FACS^® analysis of β1, αv, and α5β1 integrin expression on control clone 6, and antisense clone 31 which has a markedly reduced caveolin level. B and C were performed three times with similar results. (D) Photographs of phase images (a and b) and paxillin immunostaining (c and d) of clones 6 and 31 after plating on fibronectin and subsequent induction with CdCl₂.