Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Mar 22;144(6):1285–1294. doi: 10.1083/jcb.144.6.1285

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Caveolin is required for uPAR/β1 integrin complexes and uPAR-dependent adhesion to vitronectin. (A) The bottom panel shows uPAR/293 cells expressing varying levels of antisense caveolin (clones m1–m5) tested for adhesion to vitronectin as described in Materials and Methods. The corresponding decrease in caveolin levels for each of the clones, as judged by densitometry of immunoblots, was as follows: m1, 0% decrease; m2, 83%; m3, 89%; m4, 96%; and m5, 73%. The top panel shows β1 integrins of uPAR/293 cells cotransfected with antisense caveolin (clones m1–m5) immunoprecipitated and immunoblotted for relative amounts of uPAR as quantified by densitometry. (B) Immunoprecipitates of β1 integrins sequentially immunoblotted for caveolin, Src, and cortactin as in Fig. 4. (C) uPAR-expressing or uPAR and caveolin–coexpressing 293 cells were detached and allowed to adhere on vitronectin (Vn) fibronectin (Fn)-coated 96-well plates. After washing, the percentage of adherent cells was quantitated by spectrophotometric absorbance of stained cells. Adhesion assays were performed three times with identical results.