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. 1999 Mar 22;144(6):1097–1112. doi: 10.1083/jcb.144.6.1097

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Proteolytic processing of Nup98 is essential for NPC targeting. HeLa cells were transiently transfected with: (A–C) wild-type Nup98, (D–F) mutant Nup98 (F863S/Y866R). Localization of the transiently expressed proteins was detected by indirect immunofluorescence and confocal microscopy using an anti-myc epitope antibody followed by a Cy3-conjugated secondary antibody (A and D). Cells were also double labeled with an antibody specific for Nup358, which was detected using a secondary antibody conjugated to FITC (B and E). Cy3 and FITC images were merged to identify coincident labeling of NPCs (C and F). Bar, 10 μm.