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. 1999 Mar 22;144(6):1097–1112. doi: 10.1083/jcb.144.6.1097

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Identification of a complex containing Nup96, Nup107, and Sec13. Rat liver nuclei were fractionated as described in Materials and Methods. A fraction containing soluble NPCs was loaded onto a 10–40% sucrose gradient and centrifuged. Gradient fractions were collected and analyzed by SDS-PAGE followed by silver stain analysis (top), or immunoblot analysis with antibodies specific for Nup96 and Nup107 (bottom). Fractions, numbered from the top of the gradient, are indicated. Asterisks between lanes 5 and 6 (top) indicate proteins that sediment as a complex. Some of the proteins comprising this complex are indicated on the right.