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. 1999 Mar 22;144(6):1097–1112. doi: 10.1083/jcb.144.6.1097

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Alternatively spliced mRNAs code for Nup98 and for a Nup98-Nup96 precursor. (A) Schematic representation of Nup98 and Nup98-Nup96 precursor mRNAs. The 5′-UTRs of both mRNAs are identical except for the first 17 nucleotides, which are absent in the Nup98-Nup96 mRNA. The Nup98 coding regions are identical, except that the Nup98-Nup96 mRNA does not contain the last 18 nucleotides coding for the COOH-terminal 6 amino acids of Nup98. The Nup98-Nup96 mRNA has an extended coding region (shown in black) that generates Nup96. Both mRNAs have different 3′-UTRs. (B) Northern blot analysis of Nup98 and Nup98-Nup96 precursor mRNA transcripts. Poly(A)⁺ RNA was purified from HeLa cells, separated by agarose gel electrophoresis, and transferred to nitrocellulose membranes. Membranes were probed with a Nup98-specific probe (lane 1), or a Nup96-specific probe (lane 2). Approximate size markers (in kb) are indicated on the left.

Alternatively spliced mRNAs code for Nup98 and for a Nup98-Nup96 precursor. (A) Schematic representation of Nup98 and Nup98-Nup96 precursor mRNAs. The 5′-UTRs of both mRNAs are identical except for the first 17 nucleotides, which are absent in the Nup98-Nup96 mRNA. The Nup98 coding regions are identical, except that the Nup98-Nup96 mRNA does not contain the last 18 nucleotides coding for the COOH-terminal 6 amino acids of Nup98. The Nup98-Nup96 mRNA has an extended coding region (shown in black) that generates Nup96. Both mRNAs have different 3′-UTRs. (B) Northern blot analysis of Nup98 and Nup98-Nup96 precursor mRNA transcripts. Poly(A)⁺ RNA was purified from HeLa cells, separated by agarose gel electrophoresis, and transferred to nitrocellulose membranes. Membranes were probed with a Nup98-specific probe (lane 1), or a Nup96-specific probe (lane 2). Approximate size markers (in kb) are indicated on the left.